Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANQTPQAMRRIPVSDIEQRVKKAVAEQLGLKAEEIKNEASFMDDLGADSLDLVELVMSFENDFDITIPDEDSNEITTVQSAIDYVTKKLG 3EJE Chain:G ((18-92)) -----------SHMSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELVMALEEEFDTEIPDEEAEKITTVQAAIDYI-----

General information: TITO was launched using: RESULT: Template: 3EJE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 242 -28798 -119.00 -383.97 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain G : 0.88 3D Compatibility (PKB) : -119.00 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.663

(partial model without unconserved sides chains): PDB file : Tito_3EJE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EJE-query.scw PDB file : Tito_Scwrl_3EJE.pdb: