Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------MKNKNIGCWLLLAGSSSVCA--MQPLDDQSLAAATGQNGLTLGIQADQ--VKFNQVALIDTNGIASTSYNSKAGLIIAGNSTNPVPGIEFIKAAVSTNPSFNIAID---------TDAGGGNPFLNLAVTMGSDVNGIRLLPFSVYLAPSTSLPSPSDYALTSYAPKSI-----FSSGTTVNTGVKELIRSTGN-----LDINFVQTNKPRLNIQLGHAAQ--SVMVKFGGA-------IQSICSAASGCPITLVSDNTGATFG---------FKFAGTNASTGFVLDGFYAGVDPTGLTIGNIGVSSKFDASLN---------------NVTLGNLGTQSTTTFNNLPNGSMGSFGVTGASVT----------DFKMKVSGF----- 1G8L Chain:A ((7-409)) LMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDVVSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGAPVPEGCEAVVMQEQTEQMDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAELPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGCEVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKLAIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVRTASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGNVEVGEWVEVEPFNALFG

General information: TITO was launched using: RESULT: Template: 1G8L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1666 10029 6.02 31.64 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 6.02 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.132

(partial model without unconserved sides chains): PDB file : Tito_1G8L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G8L-query.scw PDB file : Tito_Scwrl_1G8L.pdb: