Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVIALFSKNWSRTMSATIVNTTDDNFQADVLDAETPVLVDFWAGWCAPCKAIAPVLEDLSSEYAGKVKIVKVDVTSCEETAVKYNIRNIPALLLFKNGEVVAQQIGAVPRSKLVSFIDENV 2YN1 Chain:B ((2-106)) ----------------SIIHVTDDSFDQDVLKADKPVLVDFWAEWCGPCKMIAPILDEIAEEYEGKLKVAKVNIDENPETAAKYGIRGIPTLMLFKNGEVAATKVGALSKSQLKEFLDANL

General information: TITO was launched using: RESULT: Template: 2YN1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 478 -83772 -175.26 -797.83 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -175.26 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.791

(partial model without unconserved sides chains): PDB file : Tito_2YN1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YN1-query.scw PDB file : Tito_Scwrl_2YN1.pdb: