Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------------------------------------------------------------------------------------------------MFFRIFTIFLLILSTNIEAKEKLITGEKIASSYKLLQSMTIPAIYENGMVLTKYANLYKMDNQCVIVSKLDNESNFGGYEDIIYFKNGIMLRSSKQSFFSI---FLDDEAKIKSKEIKYGDFLNGKEVQKGLKEDFIQYRKKFNKYTLSKCS---------------------------------------------------------------------------------------------------------- 5EC0 Chain:A ((1-388)) MNISRMNVDFGNSMYMNLIDGYFFELPTNVVEISKEAAEGKFTSIVEDPADLKDRLLVSTVIDETERYFLVGELAEPELHNKVESHIPYVTFLAATAYYQALKGKREDNEVTIEYFQTMLPIWLLKKLDKFSEMQKRMASKFLGTHQVKVLTLGLEKELTIKVEDAACRIES-----EVARWAIKKN-FDLEDKDYAEQFKNYDVVFCDLG-----GGTDDLVLLPAGLKPPKSRDSFVSNTAPFLAHLEKLRKEKLL-EHFDSVRELEKFIYSNI-------GKTKMERRDGNTGQKFDLTDIIKKSLKEYTEIKIAQAENTFPAPKDKVYKYLYFGGVGEVLEESISVVTEERYGRDISESNHIVAEDARLLNLYGLEVLSRAEQVKKQANEKEAQ

General information: TITO was launched using: RESULT: Template: 5EC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 490 5097 10.40 39.21 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 10.40 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.172

(partial model without unconserved sides chains): PDB file : Tito_5EC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EC0-query.scw PDB file : Tito_Scwrl_5EC0.pdb: