Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAGDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAAADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVSLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVPGKSVKLAQQYSTKLVWTLEDTPNN 5LNK Chain:J ((1-175)) MMTYIVFILSIIFVMGFVGFSS----------------------------------KPSP-IYGGLGLIVSGGVGCGIVLNFGGSFLGLMVFLIYLGGMMVVFGYTTAMATEQYPEVWVSN------------------KVVLGTFITGLLMEFLMV--------YYVLKDKEVEIVFKFNGMGDWVIYDTGDSG-------FFSEEAMGIAALYSYGTWLVIVTGWSLLIGVVVIMEITRGN

General information: TITO was launched using: RESULT: Template: 5LNK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain J - contact count / total energy / energy per contact / energy per residue : 381 -12329 -32.36 -70.45 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain J : 0.62 3D Compatibility (PKB) : -32.36 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.027

(partial model without unconserved sides chains): PDB file : Tito_5LNK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LNK-query.scw PDB file : Tito_Scwrl_5LNK.pdb: