Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKIKIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALGNHGKRRPLRFDSSSRIQ 2H5X Chain:B ((1-97)) MIASVRGEVLEVALDHVVIEAAGVGYRVNATPATLATLRQGTEARLITAMIVREDSMTLYGFPDGETRDLFLTLLSVSGVGPRLAM------------------

General information: TITO was launched using: RESULT: Template: 2H5X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 356 -67509 -189.63 -784.99 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -189.63 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_2H5X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2H5X-query.scw PDB file : Tito_Scwrl_2H5X.pdb: