Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MSSDHAHYWSVFFFVLILYAYHHGIFRSSTSLQAFIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAIGFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLIC-----LVAGLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL- 5TJ5 Chain:B ((1-213)) MNKESKDDDMSLGKFSFSHFLY-YVYGLYKLFTGHGSDIN-FGKF------LLRTSPYMWA--NLGIALCVGLSVVGAAWGIFITGSSMIGAGVRAPRITTKNLISIIFCEVVAIYGLIIAIVFSSKSNLYTGYSLFWAGITVGASNLICGIAVGITGATAAISFVKILVIEIFGS--ILGLLGLIVGLLMAGKASEFQ

General information: TITO was launched using: RESULT: Template: 5TJ5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 581 -76368 -131.44 -429.03 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -131.44 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.112

(partial model without unconserved sides chains): PDB file : Tito_5TJ5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TJ5-query.scw PDB file : Tito_Scwrl_5TJ5.pdb: