Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKNMVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNFAESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGSNWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD- 2BM5 Chain:A ((4-186)) -----------------------MQQWVDCEFTGRDFRDEDLSRLHTERAMFSECDFSGVNLAESQHRGSAFRNCTFERTTLWHSTFAQCSMLGSVFVACRLRPLTLDDVDFTL-----AVLGGNDLRGLNLTGCRLRETSLVDTDLRKCVLRGADLSGARTTGARLDDADLRGATVDPVLWRTASLVGARVDVDQAVAFAAAHGLCLAGG

General information: TITO was launched using: RESULT: Template: 2BM5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1106 -139299 -125.95 -765.38 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -125.95 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.344

(partial model without unconserved sides chains): PDB file : Tito_2BM5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BM5-query.scw PDB file : Tito_Scwrl_2BM5.pdb: