Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQANLDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFNTKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAK-MDGRSMFLTLAPKNDSK 2IFE Chain:A ((90-180)) -----------------------------------------------------------------------------------VIQVKEIKFRPGTDEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVESFPTKIEGRQMIMVLAPKKKQ-

General information: TITO was launched using: RESULT: Template: 2IFE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 382 -19391 -50.76 -215.45 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -50.76 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.559

(partial model without unconserved sides chains): PDB file : Tito_2IFE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IFE-query.scw PDB file : Tito_Scwrl_2IFE.pdb: