Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIKIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY-D-----NLALYEYVIDLCLSRGGTLF 3WYZ Chain:A ((35-174)) VFTKKLVLED-------PPDWGSLKEELKELRGKYG--K-VALLLVTRK--PSLIREVKS--RNLKALLYVQGGD---------------------MRINRMAIES-GVDALISPWFGRKDPG--------FD---HTLAGMAARRGVAIGFSLSPLLNANPYGRAQILRFMMKTWQLVKKYRVPRF

General information: TITO was launched using: RESULT: Template: 3WYZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 587 -60287 -102.70 -449.90 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -102.70 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_3WYZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WYZ-query.scw PDB file : Tito_Scwrl_3WYZ.pdb: