Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------MKNFNNCVVLIIITELTLF--TNIY-YIHEAQSSLIED-----WAIFMAKEGKEMFQNKG-------------------------------------------------------------------- 5AJ8 Chain:A ((158-325)) GNVYGPSTGTDLFISHSKGVFINGCADCAIYCLPIAGSAFLSNCTNCRVYVACHQLRLKGCTNLDMYVWCASTPIIEECDAMRFGPYRCWVGLLSSCTEDGKTYATHAEWVSRVGEIEDTARTEQNYVKVDDFQWVKKRASPHWCVLAREEERASTTVFGPATLPSSS

General information: TITO was launched using: RESULT: Template: 5AJ8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1 11 10.50 0.20 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 10.50 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.221

(partial model without unconserved sides chains): PDB file : Tito_5AJ8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AJ8-query.scw PDB file : Tito_Scwrl_5AJ8.pdb: