Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------MALLGWMIVALPFIWVFFPLTRPERAKDF----SLYAFLSEKKMLYFLIGFFVLIF-FSFLITSFVLTRWNNRNLYKK---------VNKSFEYEDEKLKKRQELLEEVYTERFGTKEE-RETVRFYSVSEEKNLDTTFIADLYKENGVELK--- 1TFJ Chain:A ((16-219)) EHLLRPLPADKQIETGPFLEAVSHLPPFFDCLGSPVFTPIKADISGNITKIKAVYDTNPTKFRTLQNILEVEKEMYGAEWPKVGATLALMWLKRGLRFIQVFLQSICDGERDENHPNLIRVNATKAYEMALKKYHGWIVQKIFQAALYAAPYKSDFLKALSKGQNVTEEECLEKVRLFLVNYTATIDV--IYEMYTRMNAELNYKV

General information: TITO was launched using: RESULT: Template: 1TFJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 434 -5070 -11.68 -37.55 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -11.68 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.330

(partial model without unconserved sides chains): PDB file : Tito_1TFJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TFJ-query.scw PDB file : Tito_Scwrl_1TFJ.pdb: