Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDNPEISVVPVPETEIKKITLKNDY-LEVVLLNYGARLHQIFAPDKEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWG----EHRLEKNCGSHHIHGGTKGWSFQFWEVEVFKTPSSIGVVFYLK--DEFSTYPGPITATITYRLT-KNELEMTTSASSLVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLEL-DQEKLPTGNWTKKEKLPIDFRKSPTLQEIL------ACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFSTTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSAQ 1L7J Chain:A ((2-339)) ----SIKIRDFGL-GSDLISLTNKAGVTISFTNLGARIVDWQKDGK-----HLILGFDSAKEYLEKDAYPGATVGPTAGRIKDGLVKISGKDYILNQNEGPQTLHGGEESIHTKLWTYEVTDLGAEVQVKFSLVSNDGTNGYPGKIEMSVTHSFDDDNKWKIHYEAISDKDTVFNPTGHVYFNLNGDASESVENHGLRLAASRFVPLKDQTEIVRGDIVDIKNTDLDFRQEKQLSNAFNSNMEQVQLVKGIDHPFLLDQLGLDKEQARLTL--DDTSISVFTDQPSIVIFTANFGDLGTLYHEKKQVHHGGITFECQVSPGSEQIPELGDISLKAGEKYQATTIYSLHTK

General information: TITO was launched using: RESULT: Template: 1L7J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1981 82271 41.53 254.71 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 41.53 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_1L7J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1L7J-query.scw PDB file : Tito_Scwrl_1L7J.pdb: