Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNSHHATESLSQDKKHAGKVGKMKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIHDVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDHVEFVRSLSEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDALRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIHAFIETECYICCPGLKEEGEALLRLLNYHPSH 1MIY Chain:B ((5-227)) --------------------------------FQEALGIIQQLKQHGYDAYFVGGAVRDLLLGRPIGDVDIATSALPEDVMAIFPKTIDVGSKHGTVVVVHKGKAYEVTTFKTDG---------SVTFVRSLEEDLKRRDFTMNAIAMDEYGTIIDPFGGREAIRRRIIRTVGEAEKRFREDALRMMRAVRFVSELGFALAPDTEQAIVQNAPLLAHISVERMTMEMEKLLGGPFAARALPLLAETGLNAYLPGL-----------------

General information: TITO was launched using: RESULT: Template: 1MIY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1092 -125994 -115.38 -588.75 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -115.38 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_1MIY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MIY-query.scw PDB file : Tito_Scwrl_1MIY.pdb: