Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSF-LEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERMKKSLVKLVG----EEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNVSDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAKASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVTIEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS 3OIF Chain:D ((1-256)) MNFSLEGRNIVVMGVANKRSIAWGIARSLHEAGARLIFTYAGERLEKSVHELAGTLDRNDSIILPCDVTNDAEIETCFASIKEQVGVIHGIAHCIAFANKEELVGEYLNTNRDGFLLAHNISSYSLTAVVKAARPMMTEGGSIVTLTYLGGELVMPNYNVMGVAKASLDASVKYLAADLGKENIRVNSISAGPIRTLSAKG-SDFNSILKDIEERAPLRRTTTPEEVGDTAAFLFSDMSRGITGENLHVDSGFHIT

General information: TITO was launched using: RESULT: Template: 3OIF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1388 -21653 -15.60 -86.61 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain D : 0.87 3D Compatibility (PKB) : -15.60 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.615

(partial model without unconserved sides chains): PDB file : Tito_3OIF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OIF-query.scw PDB file : Tito_Scwrl_3OIF.pdb: