Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFTKCFIINLPYEFTKFYFVCFHHKSKLRFLNYIESTNTILLIKGVFFMTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPDAHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLEVMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYGMRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGTCIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPAEIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE 2C20 Chain:F ((3-330)) -------------------------------------------------SILICGGAGYIGSHAVKKLVDEGLSVVVVDNLQTGHEDAITEGAKFYNGDLRDKAFLRDVFTQENIEAVMHFAADSLVGVSMEKPLQYYNNNVYGALCLLEVMDEFKVDKFIFSSTAATYGEVDVDLITEETMTNPTNTYGETKLAIEKMLHWYSQASNLRYKIFRYFNVAGATPNGIIGEDHRPETHLIPLVLQVALGQREKIMMFGDDYNTPDGTCIRDYIHVEDLVAAHFLGLKDLQNGGESDFYNLGNGNGFSVKEIVDAVREVTNHEIPAEVAPRRAGDPARLVASSQKAKEKLGWDPRYVNVKTIIEHAWNWHQKQPNGYEK

General information: TITO was launched using: RESULT: Template: 2C20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1885 -195362 -103.64 -595.62 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain F : 0.85 3D Compatibility (PKB) : -103.64 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_2C20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2C20-query.scw PDB file : Tito_Scwrl_2C20.pdb: