Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDVRKSLQTILLKEAISMKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKVTASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSARLDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDTLLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQLGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANGNVTLKTWDS-NDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKR------VRADLVFYLPKRVYDHAAIKLLNGNIMIE-TLEAKDIYTKS-TNGNIIVDQL-TATMLEVE-GVNGNIDIRNGNILDSIIETVNGTVTFGATAENLSVSL-VNGDVRLTIKEDNLKKVEAS-SVNGNVKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQLSTTTGSIYLKDTDK 3LYC Chain:H ((37-248)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PVSEYDCLELEGGG-VVNYTQSDAPEGLEIKTDRN------------IFEKYEFNVENHKLKIRPKKEFRKHTNFRPTEF-VTANSRNLKKLAAA-GSTHVNINSPLQAEEFEAGLAGSGIIQFHDTASFTNLKIEIAGSGDFVGHKVYCEELNGDAGSNTIVLGGTVGIAEFSIAGSGTVRAFDCT---DELECKIAGSGDIEAFVVNK--IKAEI-AGSGSVKYKG----------------------------------------------

General information: TITO was launched using: RESULT: Template: 3LYC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 1153 54631 47.38 281.60 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain H : 0.66 3D Compatibility (PKB) : 47.38 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.180

(partial model without unconserved sides chains): PDB file : Tito_3LYC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LYC-query.scw PDB file : Tito_Scwrl_3LYC.pdb: