Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPERIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASRGGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPNANGWDKPDIWMAKTIIPRPNSQ 2A4N Chain:B ((1-179)) MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPERIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASRGGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPNANGWDKPDIWMAKTIIPRP---

General information: TITO was launched using: RESULT: Template: 2A4N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 823 -92008 -111.80 -514.01 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 1.00 3D Compatibility (PKB) : -111.80 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.787

(partial model without unconserved sides chains): PDB file : Tito_2A4N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A4N-query.scw PDB file : Tito_Scwrl_2A4N.pdb: