Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETAEKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERALPFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELNDDLTVAKKYYL 5UM0 Chain:D ((74-235)) IVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAEKYGDEQVRIWRRSYDTLPPLLDKDDAFSAHKDRRYAHLPADVVPDGENLKVTLERVLPFWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDNLKVIEKFYL

General information: TITO was launched using: RESULT: Template: 5UM0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 548 -41795 -76.27 -257.99 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain D : 0.94 3D Compatibility (PKB) : -76.27 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_5UM0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UM0-query.scw PDB file : Tito_Scwrl_5UM0.pdb: