Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILVNIEGRLEKDDGTEEKKGIQKEVTIDRKANLK---SHRLYWIGRRAQDVFCSALALIILSPVMLITALAIVIDDLGGSPIFAQNKVSRNGRLFKFYKFCSMCVDAEAKLDALLSQNEMDGPVFKIKNDPRITRVGKFI 1E44 Chain:B ((1-96)) -GFKDYGHDYHPAPKTENIKGLGDLKPGIPKTPKQNGGGKRKRWTGD-------------------------------KGRKIYEWD--SQHGELEGYRASDGQHLG---------SFDPKTGNQLKGPDPKR--NIKKYL

General information: TITO was launched using: RESULT: Template: 1E44.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 359 37729 105.09 405.69 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.57 3D Compatibility (PKB) : 105.09 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_1E44.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E44-query.scw PDB file : Tito_Scwrl_1E44.pdb: