Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKKDAKIYVAGHRGMVGSAIVREL-NRQGY--NNIITRTHKELDLCRQDQVEEFFAEEKPDYVFLAAAKVGGIVANQSALADFMYDNMILEMNVIHSAWKNGCKKLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNK----QYGTDYISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDDFANLCVFLMNNYSGDETV--NAGTGKELSIKELTEIVAKIIGYEGEILWDTSKPNGTPRKLLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER 4BL5 Chain:L ((24-303)) ------RILVTGGSGLVGKAIQKVVADGAGLPGEDWVFVSSKDADLTDTAQTRALFEKVQPTHVIHLAAMVGGLFRNIKYNLDFWRKNVHMNDNVLHSAFEVGARKVVSCLSTCIFPDKTTYPIDETMIHNGPPHNSNFGYSYAK----RMIDVQNRAYFQQYGCTFTAVIPTNVFGPHDNFNIEDGHVLPGLIHKVHLAKSSG-SALTVWGTGNPRRQFIYSLDLAQLFIWVLREYNEVEPIILSVGEEDEVSIKEAAEAVVEAMDFHGEVTFDTTKSDGQFKKTASNSK--------------------------------

General information: TITO was launched using: RESULT: Template: 4BL5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 1413 -3161 -2.24 -11.66 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain L : 0.76 3D Compatibility (PKB) : -2.24 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_4BL5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BL5-query.scw PDB file : Tito_Scwrl_4BL5.pdb: