Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGILLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITSDRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVSLNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHY-LEDIRLRLSAEIG-RGLTLLEAKGGY-------------NRKSKEVLLIVVTDHEFSRMKPLIEEID-----PSAFVIVSSVAEVMGRGFTYHPIE 4OZN Chain:A ((33-137)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLVMAIIRPDKLADVKTALAE-VGAPSLTVTNVSGRGSQKSQWRGEEYTVDLHQKVKVECVVADTPAEDVADAIADAAHTGEKGDGKIFILPVENAIQVRTGK----

General information: TITO was launched using: RESULT: Template: 4OZN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 285 -9985 -35.03 -121.76 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.46 3D Compatibility (PKB) : -35.03 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.46 QMean score : 0.492

(partial model without unconserved sides chains): PDB file : Tito_4OZN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OZN-query.scw PDB file : Tito_Scwrl_4OZN.pdb: