Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIGRNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVTQPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVVNALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEVKTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMIKEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY 5ICL Chain:B ((19-352)) MIFVPNENNDPRVNLAIETYLLTEMPLDEPILLFYINEPSIIIGRNQNTIEEINKEYVDEHGIHVVRRLSGGGAVYHDHGNLNFSFIMPDDGNSFRDFAKVTQPIIQALHDLGVEGAELKGRNDLVINDMKFSGNAMYATNGRMFAHGTLMFDSDIDEVVNTLK----------------RVTNIKPFLSEDKQEMTTEEFRQEILLKIFGVDSIDQVKTYELTDQDWAAINKISEQYYRNWDWNYGKSPAFNLERRHRFPIGSIEMKMNVADGAIQEIKIFGDFFGLGEIKDVEDILTGVKYDKASLEEAIDQIDVKKYFGNIEKEDLLGLIY

General information: TITO was launched using: RESULT: Template: 5ICL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1726 -197130 -114.21 -619.90 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.95 3D Compatibility (PKB) : -114.21 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.95 QMean score : 0.680

(partial model without unconserved sides chains): PDB file : Tito_5ICL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ICL-query.scw PDB file : Tito_Scwrl_5ICL.pdb: