Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTLIIISHPTIEESLNQQFFKEASAGLPA-TWHHLESSYPDGKIDIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVL------ERAAHVLKGKELGIVLTTGVAEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQQYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQEMKGTSL 5LVA Chain:B ((3-150)) --VLVLAFHPNMEQSVVNRAFADTLKDAPGITLRDLYQEYPDEAIDVEKEQKLCEEHDRIVFQFPLYWYSSPPLLKKWLDHVLLYGWAYGTNGTALRGKEFMVAVSAGAPEEAYQAGGSNHYAISELLRPFQATSNFIGTTYLPPYVFYQ----------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5LVA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 589 -18907 -32.10 -134.09 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -32.10 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.596

(partial model without unconserved sides chains): PDB file : Tito_5LVA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LVA-query.scw PDB file : Tito_Scwrl_5LVA.pdb: