Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------MNKEMSQTIKVQK--MIDDLTHGI------DSQADKIIKELQGQKVKDAKML-LKTINFEMN--PTKRKLADVLEEKLASAINEQELLFETDTFNS-- 1Q48 Chain:A ((1-134)) MAYSEKVIDHYENPRNVGSLDKKDSNVGTGMVGAPACGDVMQLQIKVDDNGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLEEAGAIKNSQIAEELELPPVKVHCSILAEDAIKAAIADYKAKQGLEHHHHHH

General information: TITO was launched using: RESULT: Template: 1Q48.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 247 21267 86.10 250.19 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 86.10 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_1Q48.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Q48-query.scw PDB file : Tito_Scwrl_1Q48.pdb: