Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKFRNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYA--D-YDNFYIYFY-LESVGEYGD 5A20 Chain:F ((9-104)) ---------------ITFQSYVEQSNGEGGKT-YKWVDEFTAAAHVQPISQEEYYKAQQLQTPIGYNIYTPYDDRI----DKKMRVIYRGKIVTFIGDPVDLSGLQEITRIKGKED-----

General information: TITO was launched using: RESULT: Template: 5A20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 373 -9774 -26.20 -106.24 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain F : 0.63 3D Compatibility (PKB) : -26.20 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.072

(partial model without unconserved sides chains): PDB file : Tito_5A20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5A20-query.scw PDB file : Tito_Scwrl_5A20.pdb: