Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIELNQVR-KEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSH---EPELPGTMYVEDKEMPHLRKGTIIKIDQEDQNGGKLYGNHA 2F9H Chain:A ((3-123)) -WKMQATVTEIGKHAIDDS-EKMIILFGETATDTLKQHAVIQSFPEKDQVTLAEGDHLKIGDTNYTITKVGSFANSNLQSIAHSTLIFADAPTDEDDVIRNGVYLTPHQLPKITIGTTIDYLV-------------

General information: TITO was launched using: RESULT: Template: 2F9H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 555 -17647 -31.80 -150.82 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -31.80 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.578

(partial model without unconserved sides chains): PDB file : Tito_2F9H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F9H-query.scw PDB file : Tito_Scwrl_2F9H.pdb: