Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSGSGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLTVKEQFE--LVDQVTSKKEDSTKIDSLLQSLDIQSLK----NSYPKDLSGGERQRVAIARALYNEPSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDGYLTEEEKKKE 1L2T Chain:B ((1-228)) ---MIKLKNVTKTYKMGEEIIYALKNVNLNIKEGEFVSIMGPSGSGKSTMLNIIGCLDKPTEGEVYIDNIKTNDLDDDELTKIRRDKIGFVFQQFNLIPLLTALENVELPLIFKYRGAMSGEERRKRALECLKMAELEERFANHKPNQLSGGQQQRVAIARALANNPPIILADQPTGALDSKTGEKIMQLLKKLNEEDGKTVVVVTHDINVARFGERIIYLKDGEVEREEK---

General information: TITO was launched using: RESULT: Template: 1L2T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1170 -940 -0.80 -4.23 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -0.80 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_1L2T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1L2T-query.scw PDB file : Tito_Scwrl_1L2T.pdb: