Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQEVKSGKVLSPSTPWTQRPVPGIEVADEQQTLKATFTEPTIECPECHALVTRTAISFNAYVCPQCDEHLRMKARDRLNWFFDNV-VAELGQEFSAKDPLKFVDSKPYPDRMREAQTKTGETEALIAMQGNLNGVDMIACAFEFDFMAGSMGTVVGDRFVKAAELAIEKRQPLICFAASGGARMQEGMLSLMQMARTSAAIQKLKDTGLPYIVVLTHPVYGGVTASLAMLGDIHIAE-PKAMIGFAGKRVIEQTVRETLE-EPFQRAEYLLD-HGVVDQIVH-RHALRDTVSRLVSKLMNLP 3IAV Chain:A ((40-260)) ------------------------------------------------------------------QHAKGKLTARERIDLLLDEGSFVELDEFARHR-STNFG----L-------DANRPYGDGVVTGYGTVDGRPVAVFSQDFTVFGGALGEVYGQKIVKVMDFALKTGCPVVGINDSGGARIQEGVASLGAYGEIFRRNTH-ASGVIPQISLVVGPCAGGAVYS-PAITDFTVMVDQTSHMFITGPDVIKTVTGEDVGFEELGGARTHNSTSGVAHHMAGDEKDAVEYVKQLLSYLPS--

General information: TITO was launched using: RESULT: Template: 3IAV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1219 -40736 -33.42 -188.59 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -33.42 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_3IAV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IAV-query.scw PDB file : Tito_Scwrl_3IAV.pdb: