Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----WFKPNITREQAIELLRKE--EPGAFVIRDSSSYRGSFGLALKVQEVPASAQSRPGEDSNDLIRHFLIESSAKGVHLKGADEEPYFGSLSAFVCQH------------------ 1ZFP Chain:E ((1-98)) KPHPWFFGKIPRAKAEEMLSK-QRHDGAFLIRESESAPGDFSLSVKFG---------------NDVQHFKVLRDGAGKYFLW-V--VKFNSLNELVDYHRSTSVSRNQQIFLRDIEQ

General information: TITO was launched using: RESULT: Template: 1ZFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 231 14670 63.50 198.24 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain E : 0.72 3D Compatibility (PKB) : 63.50 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.593

(partial model without unconserved sides chains): PDB file : Tito_1ZFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZFP-query.scw PDB file : Tito_Scwrl_1ZFP.pdb: