Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLPKALKTKDRILQISLQLFNERGERSVTTNHIAAELGISPGNLYYHFRNKHEIIKELMHQYQVETLEMLSLPED-RPLTTNDKINYFQVLSGQLWNYRFIHRDVYHLVESNEDFKKIYPRFAGQVMQQGQRIYQAFVDAGLMKMTPSEIEALIINLWIVLTNWTNFLYMSRHISDNNHLEEKWVWQALRQMVFLEGPYLMGESRATYEQLLESLGPSDLFASLSSLKDE 3RH2 Chain:A ((2-209)) ------MKTRDKIIQASLELFNEHGERTITTNHIAAHLDISPGNLYYHFRNKEDIIRCIFDQYEQHLLLGFKPYADQKV-DLELLMSYFDAMFYTMWQFRFMYANLADILARDDTLKARYLKVQQAVLEQSIAVLNQLKKDGILQIEDERIADLADTIKMIIGFWISYKLTQSSI---ATISKASLYEGLLRVLMIFKAYSTPDSLANFDRLEQHFRS-------------

General information: TITO was launched using: RESULT: Template: 3RH2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 726 -54541 -75.12 -263.48 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -75.12 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.585

(partial model without unconserved sides chains): PDB file : Tito_3RH2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RH2-query.scw PDB file : Tito_Scwrl_3RH2.pdb: