Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------------------------------------------------------PTPVPGI-FDIFKKPVPTSCKLALTNGNKREVDAMLLPPSGTTILSDTSGAGVFT--AKVNSKCEFTSVSPALPTGFKIEGSVDKEGKPPKSSTKPDTAAGTKPSTKPSTKTGSKTGTKTGTKAQA-------------------- 4GEK Chain:A ((32-261)) WTFDERVAEVFPDMIQRSVPGYSNIISMIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHIDAYKAPTPVDVIEGDIRDIAIENASMVVLNFT------LQFLEPSERQALLDKIYQGLNPGGALVLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARLHKAGFEHSELWFQCFNFGSLVALKAEDAA

General information: TITO was launched using: RESULT: Template: 4GEK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 531 20235 38.11 172.94 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 38.11 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.122

(partial model without unconserved sides chains): PDB file : Tito_4GEK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GEK-query.scw PDB file : Tito_Scwrl_4GEK.pdb: