Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------KWRCNVKIYNDKRKYQGQ-ASDDWGETLTIRGY-TCYTDSY-CKASCNGLPAGWTAEGTQLN-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 3WUW Chain:G ((16-301)) KPFLSAWPSAVVPRGGHVTLRCHYRHRFNNFMLYKEDRIHIPIFHGRIFQESFNMSPVTTAHAGNYTCRGSHPHSPTGWSAPSNPVVIMVTGNHRKPSLLAHPGPLVKSGERVILQCWSDIMFEHFFLHKEGISKDPSRLVGQIHDGVSKANFSIGPMMLALAGTYRCYGSVTHTPYQLSAPSDPLDIVVTGPYEKPSLSAQPGPKVQAGESVTLSCSSRSSYDMYHLSREGGAHERRLPAVRKVNRTFQADFPLGGTYRCFGSFRHSPYEWSDPSDPLLV

General information: TITO was launched using: RESULT: Template: 3WUW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 163 4181 25.65 70.86 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain G : 0.53 3D Compatibility (PKB) : 25.65 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.258

(partial model without unconserved sides chains): PDB file : Tito_3WUW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WUW-query.scw PDB file : Tito_Scwrl_3WUW.pdb: