Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGQLTDASVVLRFGYQAIRRAGLPTEEILTKAGVALNQVDTNARTPLSAQYAFWTAAQEVSKDPDIGLHLGEHLPLYRGQVIEHLFNSSETFGEGLKRALAYQRLISDAFDAKLVVEDGRCYLTNGEQVGADNLVNRHFSECAISGVLRFFKFITEGQFHPIFIDFNFSEGASEDEYFRVYGCPVSLGQKETRLYFDPAILDFQLWQAEPELLQLHEQLAIEKLQELARYDLVGEVRRAIGSTLESGETTLETVAAQLNITPRRLRTQLSEANTSFQQILSDYRCRLAKKLLANTNESVERIVYLTGFSEPSTFYRAFKRWTNETPVEYRKRKQHR 5SUW Chain:A ((168-272)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LDAMEKISCLVKSDITRN-WRWADICGELRTNRMILKKELESRGVKFRELINSIRISYSISLMKTGEFKIKQIAYQSGFASVSYFSTVFKSTMNVAPSEYLFMLTG-

General information: TITO was launched using: RESULT: Template: 5SUW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 354 -41379 -116.89 -394.08 target 2D structure prediction score : 0.80 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -116.89 2D Compatibility (Sec. Struct. Predict.) : 0.80 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.712

(partial model without unconserved sides chains): PDB file : Tito_5SUW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5SUW-query.scw PDB file : Tito_Scwrl_5SUW.pdb: