Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTAQIELDELSVGADYEKVANRFRPIFE-KIAQGAIQREKERILPFEPIQWLKEAKLGAVRIPRKYGGDGVSLPQLFQLLAELAEADSNIVQALRGHFAFV-EDRLVAHKEHSQEVWFQRFVQGDLVGN-AWTEVGNVQIGDVVTRVTKDASGNLVVNGEKYYSTGSIFADWIDLFAYDEVN--DRHVIAAIYRHETGVSVIDDWDGFGQKTTGSGTLKVHQVHLPASHLIPFDQRFKYQ---TAFYQVVHLATLTGIARAAVETFSQEIRERKRIFSHGNGDLVRHAPQVLQVVGKASAQAYASEVITLKTAEALQKAYESHFAESEVKEHQFNVDAELESAQGQVVISNLVLDLTSQLFNALGASASSQVKQLDRFWRNARTVSSHNP-LIYKEKVIGDWEVNRTDLPFVWQIGASPRAKSA 3II9 Chain:C ((17-394)) ----------QQLADDERMVRDAAHAYAQGKLAPRVTEAFRHETTDAAIFREMGEIGLLGPTIPEQYGGPGLDYVSYGLIAREVERVDSGYRSMMSVQSSLVMVPIFEFGSDAQKEKYLPKLATGEWIGCFGLTEPNHG---SMVTRARKVP-GGYSLSGSKMWITNSPIADVFVVWAKLDEDGRDEIRGFILEKGCKGLSAPAIHGKVGLRASITGEIVLDEAFVPEENILPHVKGLRGPFTCLNSARYGIAWGALGAAESCWHIARQYVLDRKQF-----GRPLAANQLIQKKLADMQTEITLGLQGVLRLGRMKDEGT----------------AAVEITSIMKRNSCGKALDIARLARDMLGGNGISDEFGVARHLVNLEVVNTYEGTHDIHALILGRAQTGIQA----------------

General information: TITO was launched using: RESULT: Template: 3II9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2051 9751 4.75 26.71 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : 4.75 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_3II9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3II9-query.scw PDB file : Tito_Scwrl_3II9.pdb: