Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLSLKSLQCFVTLVKTKSFTRTAEELYLTQPTISKILQQLEEQLQVQLLVKPDHGRKRQIELTEIGERIYQHAGELLQAEQNIFLEIENYQQLKTGTLKLGVPPL-GSQLLTTALFDFHRQWPDIELAFMEVGSRGIEQALLNNELDVGVLLQPFDEQTFNSIELCN-YPLMVLLRRDATWATRKKINLEELQHQSFLMFPENFSLNSIILDACKQHGFYPTIACRTSQWHLLADMVLQRMGIALLPQYYTDM---LDPTLFAAVPLEKPNIQWHLVMAWKKNLPVSPAVQAWLSIVRQHFQHIKP 3FZV Chain:D ((4-300)) -YTLRQLKYFVTTVEC---AEASRKLYIAQPSISTAV--LEESF-----------------LTPAGARFYRKAQELLRMAHEFEQ-----NDVIAGQIDIGCFETVAPLYLPGLIAGFRQAYPGVEIRIRDGEQQELVQGLTSGRFDLAFLYEHDLDSTIETEPLMPPQRPHALLPEGHRFAGQAQVSLRDLCLEPMILLDVQ-PSRTYFVSLFEELGLTPNIAFSSPSIEMVRGMVGQGFGFSLLVTRPHSECTYDGK-KVVMVDLAEPVSTSGLAAAWLKRAQLTKPARLFVDYCREQLGK---

General information: TITO was launched using: RESULT: Template: 3FZV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1175 -51743 -44.04 -193.07 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain D : 0.74 3D Compatibility (PKB) : -44.04 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_3FZV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FZV-query.scw PDB file : Tito_Scwrl_3FZV.pdb: