Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDFIKVAAAVIKKDDLYLCARRKENKYKYLSKKFEFPGGKVESGETLQEALVREIYEELGVKVCINNELKKVQHEYPDFKVEITFFSCNFVGNYQYVNFDHEEIIWLPAAELALLDWAAADLPIVDLLQQI 3FFU Chain:B ((21-102)) --WIPVVAGFLRKDGKILVGQRPENNS--LAGQWEFPGGKIENGETPEEALARELNEELGIEAEVGELKLACTHSYGDVGILILFY---------------------------------------------

General information: TITO was launched using: RESULT: Template: 3FFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 280 -34178 -122.06 -416.80 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -122.06 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.462

(partial model without unconserved sides chains): PDB file : Tito_3FFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FFU-query.scw PDB file : Tito_Scwrl_3FFU.pdb: