Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAIYIGVMTGTSMDGVDIV-AASFEPLQLHATLTVPFEPELRDELMALTLPDDNEIDRMGKADVALAKMIGHGINSLIEKHQLDRSQIKAIGSHGQTIRHRPEHGFTLQIGDPNIITEITQIPVVSDFRRRDMAAGGQGAPLVPAFHQALFQHPSIHRVILNLGGIANVSMLPANNPDGVFGFDTGPANILMDAWCHRHTGHPYDENGDWAAYGHPIRSLLDRLYAHEYFSKEPPKSTGREDFNIDWLDDQLIDWRNDLTYDELEDTPENIQANLLKLTVRAIQKAIYRSNMETGEVYVCGGGAYNSYLLEQLRWRLRKHNWSVQTTDVLGLSPTWVEATAFAWLAMRFVDGLSGNLPAVTGASDFRILGTITAV 4MO5 Chain:C ((4-360)) ----YLGLMSGTSLDGMDIVLIEQGDRTTLLASHYLPMPAGLREDILALCVPGPDEIARAAEVEQRWVALAAQGVRELLLQQQMSPDEVRAIGSHGQTIRHEPARHFTVQIGNPALLAELTGIDVVADFRRRDVAAGGQGAPLVPAFHQALFGDDDTSRAVLNIGGFSNVSLLSPGKP--VRGFDCGPGNVLMDAWIHHQRGEHFDRDGAWAASGQVNHALLASLLADEFF------------FNLPWLQEHLARHPALPAAD--------IQATLLELSARSISESLLDAQPDCEEVLVCGGGAFNTALMKRLAMLMPEAR--VASTDEYGIPPAWMEGMAFAWLAHRFLERLPGNCPDVTGALGPRTLGAL---

General information: TITO was launched using: RESULT: Template: 4MO5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1930 -70782 -36.67 -205.76 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain C : 0.85 3D Compatibility (PKB) : -36.67 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_4MO5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MO5-query.scw PDB file : Tito_Scwrl_4MO5.pdb: