Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPNSQAKKGLFQFYPHNLIWLGLSVLAIVLDQWTKWIASTHMNYADPVPVLPFLNWTLLHNYGAAFSFLSDAGGWQRYFFTS--LAGLVSILFVFWLLRMPKKMVVLPVAIALILGGALGNLIDRITLGYVVDFIHVYYQNHHFPAFNIADSAITLGTILLLIDTFFLEKQRPKNSDA 2JTD Chain:A ((1-122)) -----------------------------EEEMKRLLALSQEHKFPTVPTKSELAVEILEK------------GQVRFWMQAEKLSSNAKVSYIFNEKEIFE-------------GPKYKMHIDRNTGIIEMFMEKLQDEDEGTYTFQIQDGKATGHSTLVLIGDVYKKLQKEAEF--

General information: TITO was launched using: RESULT: Template: 2JTD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 493 -34575 -70.13 -288.13 target 2D structure prediction score : 0.28 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -70.13 2D Compatibility (Sec. Struct. Predict.) : 0.28 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.139

(partial model without unconserved sides chains): PDB file : Tito_2JTD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JTD-query.scw PDB file : Tito_Scwrl_2JTD.pdb: