Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLLRLNALSPNFQLPQTAVTIGNFDGVHLGHQAMIAQLKKIAAAQGLKTLVMIFEPQPLEFFKGYDAPPRINSLREKVEYLAELGVDYIAVAKFDQHFRSMSASEFAD-LLKDKLNAQALVLGDDFHFGKDRQGNSEFLKNYGFQVTNLHTIEL---EGERVSSTRIRQVLQVGNLALAAKLLGRPYSITGRVQYG-DQIGRTLDFPTINVRLNRHKPCLNGIYGVEVICETTSLTQKVLQDNPEKPGIAGYHENSLYGAGHVGTRPAIQQEHPEWRLEVHFPDVSANLYGLFMRVTFLHYLHGELNYPSLEALKAGIDNDVQQLRQYRNDTTQFPF 5FO0 Chain:B ((14-315)) --------------LDNSAVTIGVFDGVHRGHQKLINATVEKAREVGAKAIMVTFDPHPVSVFLPRRAPLGITTLAERFALAESFGIDGVLVIDFTRELSGTSPEKYVEFLLEDTLHASHVVVGANFTFGENAAGTADSLRQICQSRLTVDVIDLLDDEGVRISSTTVREFLSEGDVARANWALGRHFYVTGPVVRGAGRGGKELGFPTANQYF--HDTVALPADGVYAGWLTILPTEAPVSGNMEP-------EVAYAAAISVGTNP----E--QRSVESFVLDRDADLYGHDVKVEFVDHVRAMEKFESVEQLLEVMAKDVQKTR-----------

General information: TITO was launched using: RESULT: Template: 5FO0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1569 -26955 -17.18 -91.99 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -17.18 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_5FO0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FO0-query.scw PDB file : Tito_Scwrl_5FO0.pdb: