Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHPFFQELQQGSQKLGLSLSD--EALTLLLKY-QDALVLWNKAYNLTAIRDPKEMLVKHLLDSLSILKDLPAG----RLLDVGTGGGMPGMIIALCQPERSCVLLDSNGKKIRFLKQFIADLKLKNVIAVQTRVENQDTIDELGQFDVITSRAFASLTDFVGAAR---PYLHEQSIIAAMKGLIPVEEMEELKQEFS------CKVIELHVPRLDEQRHLLLLQRI 3G8A Chain:F ((20-228)) -------LLEGGKALGLDLKPHLEAFSRLYALLQEA--------------GEEEVVVKHFLDSLTLLR-LPLWQGPLRVLDLGTGAGFPGLPLKIVRPELELVLVDATRKKVAFVERAIEVLGLKGARALWGRAEV--LAREAGHREAYARAVARAVAPLCVLSELLLPFLEVGGAAVAMKGPRVEEELAPLPPALERLGGRLGEVLALQLPLSGEARHLVVLEK-

General information: TITO was launched using: RESULT: Template: 3G8A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 864 -7514 -8.70 -41.06 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain F : 0.73 3D Compatibility (PKB) : -8.70 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.166

(partial model without unconserved sides chains): PDB file : Tito_3G8A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G8A-query.scw PDB file : Tito_Scwrl_3G8A.pdb: