Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKHIVIPARFSSSRLPGKPLLLIHDRPMILRVVDQAKKVEGFDDLCVATDDERIAEICRAEGVDVVLTSADHPSGTDRLSEVARIKGWDADDIIVNVQGDEPLLPAQLVQQVAKLLVDKPNCSMSTLCEPIHALDEFQRDSIVKVVMSKQNEALYFSRATIPYDRDSAKQAEPTLHSQAFRHLGLYAYRVSLLQEYVTWEMGKLEKLESLEQLRVLENGHRIAIAVAEANLPPGVDTQADLDRLNNMPVESFE 3K8D Chain:C ((21-259)) ---VIIPARYASTRLPGKPLVDINGKPMIVHVLERARE-SGAERIIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEPMIPATIIRQVADNLAQR-QVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATIPWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYGEKIHVAVAQEVPGTGVDTPEDLERV---------

General information: TITO was launched using: RESULT: Template: 3K8D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1304 -147121 -112.82 -615.57 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain C : 0.85 3D Compatibility (PKB) : -112.82 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_3K8D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3K8D-query.scw PDB file : Tito_Scwrl_3K8D.pdb: