Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFSQELWQRNLNLYQKILDLPFNQELANGTLDKEAFCHYVIQDAQYLVAYGRVLAVAAAKAFDADDIMQFSDAAKIAIVVERSLHDDFMKNFGVTKEEFKNTPLTLAGHHYTSFLTATAWSESYPVVLAALLPCFWIYAEVGKDIVSKSVPNNP-YQAWIDTYAGEEFHTAVRNVIATVDKVAARCDADTLEKMHAAYTMGAKLEWLFWDSAYHQRQWLGLDQI 1YAK Chain:C ((30-245)) --FSEECRSAAAEWWEGSFVHPFVQGIGDGTLPIDRFKYYVLQDSYYLTHFAKVQSFGAAYAKDLYTTGRMASHAQGTYEAEMALHREFAELLEISEEERKAFKPSPTAYSYTSHMYRSVLSGNFAEILAALLPCYWLYYEVGEKLL-HCDPGHPIYQKWIGTYGGDWFRQQVEEQINRFDELAENSTEEVRAKMKENFVISSYYEYQFWGMAYRKEGW------

General information: TITO was launched using: RESULT: Template: 1YAK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 930 -95193 -102.36 -442.76 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain C : 0.74 3D Compatibility (PKB) : -102.36 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.623

(partial model without unconserved sides chains): PDB file : Tito_1YAK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YAK-query.scw PDB file : Tito_Scwrl_1YAK.pdb: