Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLAIASALLSALAVSGAANAYQAEVGGSYNYLDPDNGSSVSKFGVDGTYYFNPVQTRNAPLAEAAFLNRASNVNAHVNYGDNSGTKDTQYGVGVEYFVPNSDFYLSGDVGRNEREIDNTNIDSKVTTYAAEVGYLPAPGLLLALGVKGYDEKD--GKD-GADPTVRAKYVTQVGQHDVNLEAYGAF----------GDLDEYKVRGDYYIDKTLSLGVDYYNNDLT----DKDEFGINAKKFLNQQVSVEGRVGFGD-----------------------NDNTYGVRAAYRF 2FGQ Chain:X ((148-332)) --------------------------------------------------------------------------------------------------------FNAGFGYAFDEKQTIG-TADSVGRYIGGYVAYDNG-PLSASLGLAQQKTAVGGLATDRDEITLGASYN--FGV--AKLSGLLQQTKFKRDIGGDIKTNSYMLGASAPVGGVGEVKLQYALYDQKAIDSKAHQITLGYVHNLSKRTALYGNLAFLKNKDASTLGLQAKGVYAGGVQAGESQTGVQVGIRHAF

General information: TITO was launched using: RESULT: Template: 2FGQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 589 8138 13.82 56.12 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain X : 0.60 3D Compatibility (PKB) : 13.82 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.006

(partial model without unconserved sides chains): PDB file : Tito_2FGQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FGQ-query.scw PDB file : Tito_Scwrl_2FGQ.pdb: