Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDVIKDLSELRLSYEQGELYETQVASNPHEQFLGWFNHALAANLHE-PYAMSLATAS-ASGRPHVRTVLLRGATEAGYDFYTNY-DSQKGIDLAENPYAELLFYWPSLERQVRVGGHVVKIPEQESTDYYHKRPRDSQIAAYISTPQSGKIESRELLQQRFQDLQQQVQSHEVLDKPEFWGGYRLQPDYYEFWQGRPNRLHDRLSYEKI--DGQWTLHRLMP 1CI0 Chain:B ((25-228)) ------------------TLNEKQLTDDPIDLFTKWFNEAKEDPRETLPEAITFSSAELPSGRVSSRILLFKELDHRGFTIYSNWGTSRKAHDIATNPNAAIVFFWKDLQRQVRVEGITEHVNRETSERYFKTRPRGSKIGAWASR-QSDVIKNREELDELTQKNTERFKDAEDIPCPDYWGGLRIVPLEIEFWQGRPSRLHDRFVYRRKTENDPWKVVRLAP

General information: TITO was launched using: RESULT: Template: 1CI0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 859 14717 17.13 73.95 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : 17.13 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.404

(partial model without unconserved sides chains): PDB file : Tito_1CI0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CI0-query.scw PDB file : Tito_Scwrl_1CI0.pdb: