Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDLVSRIQNLPIGKFHYTLLWVVGLGWMFDAMDTGIIAFIMTTLVKDW----SLTPVESGWIVSIGFVGMAIGAVCSGALADRFGRKTVFAMTMAIYSIATALCAFAPD-------LKWLLIFRFIVGLGLGGQLPVAVTLVSEYVPAHVRGRFIVLLESFWGLGWLVAALISYFIIPKFG--------------WHIAFLIGGLPLIYILVIWKKVPESIPYLINRGRIEEAHALVQKLEAEACVQIVHHIEVVPVAMRQKVSFKQLWSGQFA-RRSLMLWLIWFGIVYSYYGIFTWLPSLLVKQGYDIV----------KSIEYVLLMILAQLPGYLAAAWFVERLGRKITLAAFIGFCALSAYFFGQ----ADSVNSIMFWGCLMSFFNLGAWGVLYTYTPEQYPANIRAFGSGWASAMGRIGGIVAPMVVTHMMVAKNGFNHVFMMFTVVLLAVALVILILGEETQGKRLESIGL 4J05 Chain:B ((32-505)) --------------PQIKLVLLAGVGFFLDAYDLFIINQVAPMLAQVYFPKTGLPAQRQDLMKAAANIGCVVGQVMFGVLGDSFGRKFVYGKELILIIVATIFQMSAPSHWDGNRVLTWITICRVFLGIGIGGDYPMSATVVSDRANIHRRGTLLCFIFANQGWGSFVGSLVTIVTISGFKHRLKSGHTHDVDKAWRILIGLSLIPAFGTLYQR-----------------------------------------------WQEFVAYFSTWNHFRNLLGSMLGWFLVDIAFYGINLNQSVVLAQIGFAGKTGDVYDKLFQLATGNIIVTALGFLPGYYFTLFLIDIVGRKKLQFMGFIMSGLFLAILAGEIDHIGKGPLLACFTFMQFFFNFGANTTTFIVAAELFPTRIRASAHGISAAAGKCGAILSSLVFNQLKAK-IGTSAVLWIFFSTCILGFIS-TFLIDETM---------

General information: TITO was launched using: RESULT: Template: 4J05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1800 -237190 -131.77 -646.29 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -131.77 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.348

(partial model without unconserved sides chains): PDB file : Tito_4J05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J05-query.scw PDB file : Tito_Scwrl_4J05.pdb: