Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGQDHIEQHRRYI--------VISYAFMFLALFTVIFAAFAYLVARKVAVVDDAEVWIHA---HALWIMRNGILFLLMSVFAVAWFIPLFFFAWDSNLWVTASTVAGVVFSAIAWLFLLNAWLKGLSKYLKNKAVF 3J9O Chain:A ((2-135)) -AKNKIPNSRLMINYETNVDGVLKKKELPYRVLVVGDLSKGRSVDAKKEFADREVRRVNNGVDRVLEEMNISFDFEAPNFVSKDPSNLKVNYRIESVKDFRPDAVAKKV-PEIRALLEMKEILASFAKDIENNRNL

General information: TITO was launched using: RESULT: Template: 3J9O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 239 -9424 -39.43 -76.61 target 2D structure prediction score : 0.23 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -39.43 2D Compatibility (Sec. Struct. Predict.) : 0.23 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.167

(partial model without unconserved sides chains): PDB file : Tito_3J9O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3J9O-query.scw PDB file : Tito_Scwrl_3J9O.pdb: