Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIYILGSAAGGGCPQWNCNCPNCHGVRTGTINAKVRTQSSIAISENGVDWILLNASPDIRQQLFDFKAAQPARKLRDTGITNVILMDSQLDHTTGLLTLREG--CPMNVWCTEMVYQDLTTGFPVFNMLKHWNGGLQY-HQVDPKQAFIIDGFENLEFLPLIIQSAAPPYSPHRHDPHEGDNIALIIKDHKTQKQLFYAPGLGKIDDQIMQIMQ--DSDCVMIDGTLWTDDEMQQTGVGKKTGREMGHLYISGEGGSLSYLNQLSTPKKVLIHINNTNPILNEDSAQFAELKANGVEVAFDGMQIEL 3G1P Chain:B ((3-251)) LTLTLTGTGGAQGVPAWGCECAACARARRSP--QYRRQPCSGVVKFND-AITLIDAGLHDLAD---R--W------SPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPPDEQGCDDLFKHP----G----LLDFSHTVEPFVVFD---LQGLQVTPLPLNHSK-------------LTFGYLLETA--HSRVAWLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRAD-----------APRNHCDLN---TVLALNQVIRSPRVILTHISHQFDAW-----LMENALPSGFEVGFDGMEIGV

General information: TITO was launched using: RESULT: Template: 3G1P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1446 -43608 -30.16 -178.72 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -30.16 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.400

(partial model without unconserved sides chains): PDB file : Tito_3G1P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G1P-query.scw PDB file : Tito_Scwrl_3G1P.pdb: