Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKDERIIQNQDNKKTIRHEDYIAGIGKGMAILDSFSSNQHRLKISMAAEKTGLTRAAARRHLLTLEYLGYLESDG-HYYYLTPKVLKFSGAYLGAAQLPKVSQPLLNLLTNQTSLIYSVMVLDGYEAITIARSAAHQQTDRVNPYGLHLGNRLPAHATSAGKILLAHLSQEEQLDWLQKYPLQRLTKYTHVENDKFLELLKEIREQDWCYSREEHELGFHALAVPIYGQDFKVVAALNIVSPTMRTTEGYLIQYILPLLQETARDLRQVL 1MKM Chain:B ((3-247)) ---------------------HMNTLKKAFEILDFIVKNPGDVSVSEIAEKFNMSVSNAYKYMVVLEEKGFVLRKKDKRYVPGYKLIEYGSFVLRRFNIRDIAHDHLVDIMKRTGETVHLILKDGFEGVYIDKVEGE----QSIPMVSRLGMKVDLYSTASGKSILAFVPEKELKEYLKIVELKPKTPNTITNPRVLKRELEKIRKRGYAVDNEENEIGIMCVGVPIFDHNGYPVAGVSISGVARKFT-EEKIEEYSDVLKEKAEEISRKL

General information: TITO was launched using: RESULT: Template: 1MKM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1154 -50855 -44.07 -208.42 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -44.07 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_1MKM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MKM-query.scw PDB file : Tito_Scwrl_1MKM.pdb: