Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTYAYVRIEPNLQFEFSRYISFFSEYGYKIQKNRLVVEEVSVDKSIVYRDKFLNLISYSLEEGDILVIKSMDCLGNSFEEILDIVNKIDQKKIRLICLDYSKSEINGDLKIFFLHFLKLSAEFEKLFKSDKKNF---KDHKPTRKVGRPEILNNEQKSRVIELFKKGYSVYSLAKEFSVTRTVIQRILNNASKTNIL 4M6F Chain:A ((2-185)) --LIGYVRVSTND-QNTDLQRNALVCAGC----EQIFEDKLSGTR--TDRPGLKRALK-RLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDS-IDTSSPMGRFFFHVMGALAEVERELIIERTMAGLAAARNKGRIGGRPPKLTKAEQEQAGRLLAQGIPRKQVALIYDVALSTLYKKHPAKRAH---

General information: TITO was launched using: RESULT: Template: 4M6F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 512 -27019 -52.77 -149.28 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -52.77 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_4M6F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M6F-query.scw PDB file : Tito_Scwrl_4M6F.pdb: